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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 9
First page : ( 1441) Last page : ( 1446)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00278.X

Validated UPLC/Q-TOF-MS Method for Simultaneous Determination of Telmisartan and Hydrochlorothiazide in Human Plasma

Khan Hamid1,*, Ali Mushir2, Ahuja Alka2, Ali Javed2

1JK College of Pharmacy, Bilaspur, Chhattisgarh, India-495001

2Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, India-110062

*Corresponding Author E-mail: khanhamid770@gmail.com

Online published on 12 January, 2017.

Abstract

In the presented work the ultra-performance liquid chromatographic/quadrupole time-of-flight mass spectrometric (UPLC/Q-TOF-MS) method has been developed for simultaneous determination of telmisartan and hydrochlorothiazide in human plasma. For identification of drugs, the Q-TOF mass spectrometer was operated in negative ionization mode and quantification was done using the MS/MS transitions at m/z 513.18 to 469.13 for telmisartan, and 268.90 to 204.94 for hydrochlorothiazide. The chromatographic separation was achieved on Acquity UPLCTM BEH C18 (100.0 × 2.1 mm, 1.7μm) column usingisocraticmobile phase consisting of acetonitrile-2mM ammonium acetate (50: 50, v/v) at a flow rate of 0.25 mL/min. The elution of telmisartan and hydrochlorothiazide was occurred at 2.25 and 1.22 min, respectively. The calibration curves were linear over the concentration range of 1–1000 ng/mL for both the drugs. The developed method was validated according to ICH guidelines. The method was applied for pharmacokinetic study of telmisartan and hydrochlorothiazide in human plasma.

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Keywords

UPLC/Q-TOF-MS, Telmisartan, Hydrochlorothiazide, Validation, Pharmacokinetic Study.

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