(34.238.189.240)
Users online: 2664    [ij] [ij] [ij] 
Email id
 

Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 6
First page : ( 725) Last page : ( 730)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00138.4

Development and Validation of RP-HPLC Method for Estimation of Epigallocatechin -3-gallate (EGCG) in Lipid based Nanoformulations

Avadhani Kiran S, Amirthalingam Muthukumar, Reddy Meka Sreenivasa, Udupa Nayanabhirama, Mutalik Srinivas*

Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences (MCOPS), Manipal University, Manipal, 576104, Karnataka State, India

*Corresponding Author E-mail: ssmutalik@yahoo.com

Online published on 21 July, 2016.

Abstract

Epigallocatechin-3-gallate (EGCG) is a potent antioxidant and free radical scavenger present in green tea. It is extensively used as antioxidant, immunomodulator, anticancer, neuroprotective, cardioprotective, antiviral, antibacterial, photo protective and anti aging agent. The objective of the present work was to develop and validate an RP-HPLC method for determination of EGCG in lipid based nanoformulations. RP-HPLC analysis was performed by isocratic elution using a Phenomenex C18 column (4.6 × 250 mm, 5 μm) at 25°C. A mixture of acetonitrile and 1% v/v acetic acid (25:75) of pH 2.8 was used as a mobile phase with a flow rate of 0.8 mL/min. The analysis was performed using a UV detector at 280 nm. The results showed that the peak area response was linear within the concentration range of 0.5–40 μg/mL (r =0.9998). The values of LOD (limit of detection) and LOQ (limit of quantification) were 0.125 μg/mL and 0.272 μg/mL respectively. The % RSD for peak area of intraday and interday precision was 0.08 and 0.2%, respectively. The results of accuracy for the different concentrations of EGCG (8, 10 and 12 μg/mL) were 100.29±0.19, 101.10±0.10 and 100.44±0.25% respectively. The validated method was successfully applied for the estimation of EGCG content in lipid based nanoformulations.

Top

Keywords

Epigallocatechin-3-gallate (EGCG), HPLC, nano-formulations, liposomes.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
625,153,253 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.