Phytochemical Composition of Methanolic Extract of Phyllanthus acidus L (Skeels) Fresh Leaves by GC/MS Analysis Phatak Rohan Sharadanand1,*, Hendre Anup Subhash2, Durgawale Pushpa Prakash2 1Directorate of Research, Krishna Institute of Medical Sciences Deemed University, Malkapur, Karad-415539, India 2Department of Biochemistry, Krishna Institute of Medical Sciences, Malkapur, Karad-415539, India *Corresponding Author E-mail: phatak.rohan1983@gmail.com
Online published on 21 June, 2016. Abstract Background Secondary metabolites and different phytochemicals are present in the medicinal plant Phyllanthus acidus belonging to family Phyllanthaceae. Scientific evidence data related GC-MS analysis of methanolic extract of Phyllanthus acidus fresh leaves has not been reported till date during literature review. Objective The study was aimed to investigate phytochemicals present in the methanolic extract of Phyllanthus acidus fresh leaves. Material and Methods Methanolic extraction of fresh leaves Phyllanthus acidus was prepared by simple maceration and GC-MS analysis data was provided by IICPT, Thanjavur. Phytochemical investigation was carried out to identify the possible components from Phyllanthus acidus fresh leaves by evaluating retention time in the GC-MS chromatogram. Results Eleven peaks have revealed in the GC-MS spectrum of the methanolic extract of Phyllanthus acidus. From the results, squalene is present as major bioactive compounds in methanolic extract of Phyllanthus acidus fresh leaves. Conclusion The present data provides to find the molecular formula and weight of 11 biomolecules. Further investigation should be done to isolate bioactive compounds and their structural elucidation and screening of pharmacological activity in the drug development. Top Keywords GC/MS analysis, Phytochemicals, Phyllanthus acidus. Top |