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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 5
First page : ( 509) Last page : ( 512)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00095.0

Simultaneous UV-Spectrophotometric Estimation of Paracetamol and Guaiphenesin by First Order derivative Method in Combined Dosage Form

Rele Rajan V.*

Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai-400016

*Corresponding Author E-mail: drvinraj@gmail.com

Online published on 21 June, 2016.


The objective of the study was to develop a simple, accurate, precise and rapid a UV spectrophotometric i.e. first order derivative method for the determination of paracetamol and guaiphenesin in combined dosage form i.e. tablets by using methanol as a solvent. The method was further validated by ICH guidelines. The proposed first order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence wavelengths 230 nm and 263 nm were selected for the estimation of paracetamol and guaiphenesin respectively. The linearity of the proposed method was found in the concentration range of 1 to 10 μg/ml (r2= 0.9998) for paracetamol and 10 to 100 μg/ml (r2= 0.9993) for guaiphenesin respectively. The percentage mean recovery was found to be 99.955% for paracetamol and 99.88% for guaiphenesin respectively. The method was also statistically validated for its linearity, accuracy and precision. Both intra and inter day variations showed less percentage (%) RSD values indicating high grade of precision of this method.



UV spectrophotometric estimation, First order derivative method, Paracetamol, Guaiphenesin Methanol.


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