Detection of pap C gene for the presence of adhesive pili among clinical isolates of E. coli Deepika V.1, Dr. Gopinath P.2 12nd Year BDS, Saveetha Dental College and Hospital, Chennai, Tamil Nadu, India 2Senior Lecturer, Department of Microbiology, Saveetha Dental College and Hospital, Chennai, Tamil Nadu Corresponding Author E-mail:
Online published on 2 March, 2017. Abstract Escherichia coli is the major causative agent for this, and is also a predominant facultative member of the normal human intestinal flora. This agent primary of UTIs accounts for greater than 80% of these infections. Virulence factors (VFs) associated with UPEC includes adhesins (P fimbriae, type 1 fimbriae, S and F1C fimbriae, afimbrialadhesin), toxins (hemolysin, and cytotoxic necrotizing factor), siderophores (the aerobactin system) and polysaccharide coatings (group II capsules). In this study we have taken 20 clinical isolates of E.coli and were subjected to antibiotic sensitivity pattern followed by the detection of papC gene by PCR. We have observed increased percentage of resistance to penicillin and cephalosporins followed by other group of drugs. 40% of our strains were found to have this gene. We conclude that papC gene is one of the important putative virulent factor for causing urinary tract infection. However, more number of isolates have to be included to validate the proper conclusion. Top Keywords Escherichia coli, papC gene, PCR. Top |