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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 11
First page : ( 1985) Last page : ( 1990)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00405.4

A stability indicating RP-HPLC assay method for simultaneous estimation of abacavir, Lamivudine, Nevirapine and Zidovudine in pharmaceutical dosage form

Nadig Sreekanth*, Dr Jacob Jane T.

Department of Pharmaceutical Chemistry, Nitte Gulbishetty Institute of Pharmaceutical Sciences, Mangalore, India

*Corresponding Author E-mail: sreekanth30@rediffmail.com

Online published on 2 March, 2017.


A stability-indicating reverse phase HPLC method has been developed and validated for the simultaneous Assay estimation of Abacavir, Lamivudine, Nevirapine and Zidovudine in pharmaceutical dosage form. The chromatographic separation was achieved on a simple Inertsil ODS C18, 250 x 4.6 mm, 5 μm (Make: GL Sciences). The method employed a simple isocratic elution mode with a ternary mixture of mobile phase. The detection wavelength was set at 270 nm. The proposed method was extensively validated as per ICH guidelines. The specificity of the method was proved by performing forced degradation studies along with peak purity. This method is found to be simple, fast and economical Hence this validated method can be used in routine quality testing of individual dosage forms and combination dosage forms of Abacavir, Lamivudine, Nevirapine and Zidovudine.



Abacavir, Lamivudine, Nevirapine and Zidovudine, Stability indicating, Forced degradation and Peak purity.


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