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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 11
First page : ( 1922) Last page : ( 1926)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00393.0

Antibacterial and antioxidant activity of ethanolic extract of Ceiba pentandra leaves and its phytochemicals analysis using GC-MS

Bhavani R.1, Bhuvaneswari E.1, Rajeshkumar S.2,*

1PG and Research Department of Biochemistry, Adhiparasakthi College of Arts and Science, Kalavai-632506, Vellore District, Tamil Nadu, India

2School of Bio-Sciences and Technology, VIT University, Vellore, Tamil Nadu, India

*Corresponding Author E-mail: ssrajeshkumar@hotmail.com, j3ssrajesh@gmail.com

Online published on 2 March, 2017.

Abstract

In this present investigation, the in-vitro free radical scavenging activity, antimicrobial activity and phytochemical evaluation of ethoanol extracts of Ceiba pentandra leaves were performed. The phytochemical characterization of leaf extract was done by GC-MS analysis. The result of screening of extract showed the presence of nine bioactive components. Among these nine compounds, Hexadecanoic acid was fatty acids. Antibacterial activity was studied against bacterial strains like Staphylococcus aureus, Streptococcus sp, E. coli, and Klebsiella pneumonia Agar well diffusion method. Ethanol extract was found to highly active against E. coli and Staphylococcus aureus in dose dependent manner. In-vitro antioxidant activity of Ceiba leaf extract was evaluated via DPPH, nitric oxide and hydroxyl assay. The free radical scavenging activity of extracts was found to be great potential comparable with standard. IC50 was calculated for antioxidant activity of leaf extract against DPPH, nitric oxide and hydroxyl are 27.4, 24.45 and 51.65 μg/ml, respectively.

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Keywords

Mucuna pruriens, Antibacterial, Antioxidant, Free Radical Scavenging, GC-MS Analysis.

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