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Research Journal of Pharmacy and Technology
Year : 2016, Volume : 9, Issue : 10
First page : ( 1629) Last page : ( 1631)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2016.00325.5

Detection of Hly-A gene for the production of alpha hemolysin in clinical isolates of Escherichia coli

Sriram Nirisha1, Dr. Gopinath P.2

12nd Year BDS, Saveetha Dental College and Hospitals, Chennai, India

2Senior Lecturer, Department of Microbiology, Saveetha Dental College and Hospitals, Chennai, India

Corresponding Author E-mail:

Online published on 2 March, 2017.


Alpha hemolysin is a toxin secreted by Escherichia coli causes cell death by binding with the outer membrane, with subsequent oligomerization of the toxin monomer and water-filled channels. These are responsible for osmotic phenomena, cell depolarization, and loss of vital molecules, leading to its demise. A total of 20 clinical isolates of E. coli were screened for the presence of hlyA gene by PCR. We have observed 60% positivity among our isolates. There are several virulent factors that are associated with urinary tract infections, production of alpha hemolysin one amongst those factors also play a crucial role in pathogenesis of UTI.



Hlya Gene, Alpha Hemolysin, Escherichia Coli, PCR, Urinary Tract Infections.


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