Anti-Bacterial Screening and Optimization of Lipids for the Preparation of Nanoemulsions Singh Neeru1,*, Verma Saurabh Manaswita2 1Division of Biomedical Lab Technology, University Polytechnic, Birla Institute of Technology, Mesra, Ranchi, Jharkhand -835215 (India) 2Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand-835215 (India) *Corresponding Author E-mail: neerumicro@yahoo.com
Online published on 30 July, 2015. Abstract The present study aimed to screen and optimized the levels of lipids and surfactants that holds an innate antibacterial activity against gram positive and gram negative bacteria. Gram positive bacteria (S. aureus and B. subtilis) were found to be more susceptible to various explored excipients as compared to Gram-negative bacteria (E. coli, P. aeruginosa). The mean particle of Smix in absence of oleylamine decreased from 300.32 ±5.64 nm to 58.43 ± 0.34 nm when cremophor RH 40 concentration increased from 0.25% w/v to 0.75% w/v. Thereafter, from 1.0% w/v to 2.0% w/v, there was insignificant change in mean particle size (10.34 ± 0.22 to 11.24 ± 0.32). Similarly, mean particle of Smix in presence of oleylamine decreased from 358.34 ± 8.32 nm to 110.45 ± 2.23 nm when cremophor RH 40 concentration increased from 0.25% w/v to 0.75% w/v. Thereafter, from 1.0% w/v to 2.0% w/v, the mean particle ranged between 54.21 ± 1.34 to 59.32 ± 1.43, illustrating insignificant variations. Top Keywords Lipids, surfactants, particle size, anti-bacterial activity. Top |