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Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 4
First page : ( 437) Last page : ( 442)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00073.6

Using GC/MS to Study the Chemical Composition of Essential Oil of Thymus vulgaris L. at AL-Qadmous Area, Syria

Moualla N.1,*, Naser M.2

1Department of Basic Sciences (Plant Biotechnology), Faculty of Pharmacy, Al-Andalus University for Medicinal Sciences, Al-Qadmous, Syria

2Department of Basic Sciences (Organic Chemistry), Faculty of Pharmacy, Al-Andalus University for Medicinal Sciences, Al-Qadmous, Syria

*Corresponding Author E-mail: mansouroussama@yahoo.fr

Abstract

Thyme is a wild plant naturally found in the AL-Qadmous area at varying altitudes up to more than 1000 m above the sea level. Essential oil of thyme dry leaves was extracted by water distillation method using Clevenger apparatus. Gas Chromatography – Mass Spectrometry technique (GC-MS) was used to determine essential oil content of Thymol, Carvacrol and Pinene compounds. The results of chemical analysis shows that Thymol percentage ranged between 2.09 and 7.80% and that of Carvacrol ranged between 12.15 and 22.58%, while Pinene content ranged between 75.32 and 80.05Statistical analysis shows significant differences between the compounds contents studied in the three sites. The highest percentage of both Thymol and Carvacrol is recorded at A2 site (560 Meter) above sea level. On the other hand, A3site (1000 Meter) above sea level has the highest value of Pinene compounds compared with the other sites.

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Keywords

Thymus vulgaris, Essential oil, Thymol, Carvacrol, Pinene.

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