Bioactivity and Molecular Docking of Secondary Metabolites produced by Streptomyces xanthochromogenes JAR5 Chauhan Ritika, Singh Nidhi, Abraham Jayanthi* Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India *Corresponding Author E-mail: jayanthi.abraham@gmail.com
Online published on 2 May, 2015. Abstract The present investigation was aimed to explore in vitro and in silico antimicrobial potential of secondary metabolites produced by terrestrial actinomycetes, S. xanthochromogenes JAR5 isolated from deep forest of Kodaikanal, Tamil Nadu, India and was confirmed by 16S rRNA gene sequencing analysis. The bioactive metabolites produced by the strain JAR5 were obtained from the optimized culture medium through solvent extraction method. The crude extract obtained was purified using silica gel column chromatography and partially purified active elute was characterized using UV, FTIR and GC-MS spectroscopic analysis. The partially purified bioactive metabolites produced by strain JAR5 showed 23.333±0.471 diameter zone of inhibition and minimum inhibitory concentration of 80 μg/ml against Enterococcus sp. In silico molecular docking studies revealed binding into crystal structure of topoisomerase II using Autodock vina 4.2 program. The molecular docking of biological active compounds is in good agreement with in vitro antimicrobial screening where the bioactive metabolites showed effective inhibitory effect against Enterococcus sp. Top Keywords Antibiotics, clinical pathogens, molecular docking, gas chromatography, minimum inhibitory concentration. Top |