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Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 2
First page : ( 207) Last page : ( 211)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00038.4

Analytical Method Development and Validation for the Determination of Allopurinol and Alphalipoic Acid Using Reverse Phase HPLC Method in Bulk and Tablet Dosage Form

Vani R.1,*, Kumar B. Vijaya2, Mohan G. Krishna3

1Research Scholar, JNTU-K, Kakinada and Deccan School of Pharmacy, Hyderabad

2Principal, Jangaon Institute of Pharmaceutical Sciences, Jangaon

3Center for Pharmaceutical Sciences, JNTU-H, Hyderabad

*Corresponding Author E-mail: vrathipelli@gmail.com


A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Allopurinol and Alpha lipoic acid in bulk and pharmaceutical dosage form using C18 column (inertsil ODS, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of 0.1 M Dipotassium Phosphate buffer (pH 3.5) and acetonitrile in the ratio of 55:45v/v. The detection was carried out at 250 nm for Allopurinol and 212 nm for Alpha lipoic acid. The method was linear over the concentration range for Allopurinol 60–140 μg/ml and for Alpha lipoic acid 60–140μg/ml. The recoveries of Allopurinol and Alpha lipoic acid were found to be 98.9% and 98.7% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.



Allopurinol, Alphalipoic acid, reverse phase HPLC, validation.


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