Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 2
First page : ( 167) Last page : ( 171)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00030.X

Isolation and characterization of drug resistant Salmonella typhi from sewage water

Swaroop Vanka Kanth, Mukherjee Aparna, Sharma Sumit, Osborne William Jabez*

Microbial Bioremediation Lab, School of Bioscience and Technology, VIT University, Vellore-632014, India

*Corresponding Author E-mail: jabez.vit@gmail.com

Abstract

Salmonella typhi (S. typhi) is a facultative intracellular pathogen, causes typhoid fever in humans. Emergence of fluoroquinolones resistant strains is one of the major public health concern, reports revealed the occurrence of drug resistant strains with increasing minimum inhibitory concentration (MIC) of Ciprofloxacin have shown fatal effects and has led to treatment failures against S.typhi. The present study was focused on isolation of drug resistant S. typhi from various sewage samples. A totally four different drug resistant isolates were obtained on SSA plates supplemented with 200 μgL⁻¹cefixime, a third generation drug used in the treatment of typhoid. The minimal inhibitory concentration was tested for the isolates and the isolate VITSSAJ3 was found to be resistant up to a concentration of 8000 μgL⁻¹.

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Keywords

Salmonella spp., Cefixime, minimum inhibitory concentration (MIC), drug resistance.

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