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Research Journal of Pharmacy and Technology
Year : 2015, Volume : 8, Issue : 11
First page : ( 1560) Last page : ( 1565)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2015.00278.4

Isolation and Characterization of Bacteriocin Producing Lactic Acid Bacteria from Diary Effluent

Indira M.1, Venkateswarulu T.C.1, Chakravarthy K.1, Reddy A. Ranganadha1, Prabhakar K. Vidya2,*

1Department of Biotechnology, Vignan's University, Vadlamudi, Guntur-522213, India

2Department of Biotechnology, Vikrama Simhapuri University, Nellore-524001, India

*Corresponding Author E-mail: kodalividyaprabhakar@gmail.com

Online published on 1 January, 2016.

Abstract

Bacteriocins are antimicrobial peptides produced by bacteria and Achaea. This study was focused to isolate bacteriocin producing bacteria from dairy effluent and the species were screened for antimicrobial and probiotic characteristics. The strains 3 and 5 confirmed highest antimicrobial activity against multidrug resistant pathogens such as Escherichia coli, Staphylococcus aureus, Streptococcus sp, Klebsiella pneumoniae, Enterobacter aerogenes, and Proteus vulgaris and negative activity was found against Bacillus subtilis. The strains showed high antimicrobial activity at neutral pH and temperatures in the range of 30–50ºC. The strains were also tested for antibiotic resistance as well as acid and bile salt tolerance tests.

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Keywords

Bacteriocins, Antimicrobial peptides, Antimicrobial Activity, Probiotics.

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