Spectrum of Antimicrobial Susceptibility of E. coli and Staphylococcus aureus Isolates from Clinical Samples Shahid S. Mohammed1,*, Dr. Umar Nawaz2 1Lecturer, Dept. of Microbiology, Khaja Bandanawaz institute of Medical Sciences, Gulbarga-585104, Karnataka 2Associate Professor, Gulbarga institute of Medical Sciences, Gulbarga-585104, Karnataka *Corresponding Author E-mail: mdshahid06@gmail
Online published on 2 December, 2015. Abstract Background and objectives: Resistance of antibiotics is increasing the public health threat and serious clinical problems in treatment. Aim: To determine the antibiogram of E.coli and Staphylococcus aureus isolates and to discuss sensitivity and resistance pattern. Methodology: The Kirby Bauer method was used to evaluate the susceptibility of 120 isolates each of E.coli and Staphylococcus aureus to various antibiotic drugs on Mueller-Hinton agar. Results: The prevalence of multiple drug resistance as determined on Mueller Hinton agar was 87.8% for E.coli and 90.55% for Staphylococcus aureus. For E.coli, resistance ranged from 7.5% (gentamicin and amikacin) to 89.16% (cotrimoxazole, ampicillin). In the case of Staphylococcus aureus resistance ranged from 15.83% (erythromycin and gentamicin) to 94.16% (penicillin). Conclusion: High levels of resistance seen in this present study. And an attempt was done to find out the most resistant antibacterial drug for routine infections. Top Keywords E.coli, antimicrobial resistance, Staphylococcus aureus, antibiotic susceptibility testing, Kirby-Bauer, Muller-Hinton agar. Top |