Hepatoprotective and Antioxidant Effects of Mucuna Pruriens Against Acetaminophen-Induced Hepatotoxicity in Albino Wister Rats
Kumar C. Hari1,,*, Dr. Ramesh A.2, Dr. Mohan G. Krishna3
1Research Scholar, Jawaharlal Nehru Technological University Kakinada, Kakinada, A.P., India
2Dept. of Pharmacology, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, A.P., India
3Centre for Pharmaceutical Sciences, JNTU-H, Hyderabad, A.P., India
*Corresponding Author E-mail: email@example.com
Online published on 27 March, 2014.
The objective of the present study was to investigate the antioxidant and hepatoprotective activity of ethanolic seed extract of Mucuna pruriens against paracetamol (PCT) induced hepatotoxicity. The material was dried in shade, they were powdered and extracted with ethanol. Preliminary phytochemical tests were done. The hepatoprotective activity of the ethanol extract was assessed in paracetamol induced hepatotoxic albino wister rats. Paracetamol (3g/kg) has enhanced the levels of various biochemical markers of hepatic damage like SGOT, SGPT, ALP, bilirubin and antioxidant levels were tested in both Paracetamol treated and untreated groups. The various biochemical and Histopathological investigations done were aspartate aminotransferase (SGOT), Alanine Transaminase (SGPT), Alkaline phosphatise (ALP), Bilirubin, antioxidant activity by DPPH (1,1-diphenyl 2-picryl hydrazyl), NBT (Nitro Blue Tetrazolium), Hyderogen peroxide (H2O2), lipid perioxidation, hyderoxil radical and nitric oxide methods. Treatment of ethanolic extract of M. pruriens seeds (100mg/kg, 200mg/kg and 400mg/kg body weight) has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner. Our findings suggested that M. pruriens ethanol root extract possessed antioxidant and hepatoprotective activity.
Mucuna pruriens, paracetamol, Hepatoprotective, Histopathological.