Phytochemical Evaluation of Tribal Drug Veerasingam Pattai Zanthoxylum tetraspermum Wight and Arn stem bark
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Zanthoxylum tetraspermum Wight and Arn stem bark (Rutaceae) known as Veerasingam pattai is traditionally used by the tribals for treating tooth ache and in tooth extraction. The present study reveals the Phytochemical Evaluation of Tribal Drug Veerasingam Pattai by HPTLC and LC-MS/MS, which is used for treating microbial infections and tooth ache. The preliminary screening of Phytochemicals and HPTLC profile of secondary metabolites from the stem bark of zanthoxylum tetraspermum Wight and Arn have shown the presence of various important secondary metabolites such as alkaloids, flavonoids, glycosides, Phenols, saponin, sterols, tannins and triterpeniods. Ethanolic and aqueous extracts were selected for LC-MS/MS analysis due to high percentage yield. In the LC-MS/MS analysis, bioactive Phytochemical Compounds like Dihydronitidine, Acetonyl Dihydro avicine, Sesamin, 8-O-Desmethyl-N-nornitidine, Oxyavicine, Dihydroacvine, Liriodenine and Savinin were identified in the Ethanolic extract and N-caffeoyl O-methylyramine, Bergenin, Tetrahydrocurcumin, Tetrahydropalmatine, Sesamolin, Fagaridine and 8-O-Desmethy-N-nornitidine were identified in Aqueous extract of Veerasingam Pattai. The generated data has been provided the basis for its therapeutic value and can be used as therapeutant.
Tribal Drug, Zanthoxylum tetraspermum, Phytochemical, HPTLC, LC-MS/MS.