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Research Journal of Pharmacy and Technology
Year : 2012, Volume : 5, Issue : 10
First page : ( 1312) Last page : ( 1315)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.

In vitro Antioxidant Activity of Ripe Pericarp of Polyalthia longifolia Thw

N. Dileep1,2, K.N. Rakesh1,2, Junaid Syed1,2, Poornima G.1,2,*, Swarnalatha S.P.1, Kekuda T.R. Prashith 1

1Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India

2Department of Biochemistry, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India

*Corresponding Author E-mail: poo.an30@gmail.com

Online published on 12 February, 2013.

Abstract

The present study examined the antioxidant activities of different solvent extracts of the ripe pericarp of Polyalthia longifolia Thw. (Annonaceae).The powdered ripe pericarp was extracted successively with ethyl acetate, chloroform and ethanol. Antioxidant activity of solvent extracts was determined by DPPH free radical scavenging assay and Ferric reducing assay. Total phenolic content was estimated by Folin-Ciocalteau method. The solvent extracts scavenged DPPH free radical dose dependently with IC50 value of 52.22±0.05, 61.82±0.01 and 06.85±0.05μg/ml for ethyl acetate, chloroform and ethanol extract respectively. An increase in the absorbance with increase in the concentration of extracts was observed in ferric reducing assay indicating reducing potential. The phenolic content of ethyl acetate, chloroform and ethanol extract was found to be 95±0.09, 85±0.01 and 224±0.10 μg Gallic acid equivalents/mg of extract respectively. Ethanol extract showed potent antioxidant activity than other extracts and this effect could be attributed to high phenolic content in ethanol extract. To our knowledge, this is the first report on antioxidant activity of solvent extracts of ripe pericarp of P. longifolia.

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Keywords

Polyalthia longifolia, Pericarp, DPPH, Ferric reducing, Total phenolic.

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