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Research Journal of Pharmacy and Technology
Year : 2011, Volume : 4, Issue : 8
First page : ( 1303) Last page : ( 1306)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.

High Performance Thin Layer Chromatographic Analysis of Paracetamol and Etoricoxib in Spiked Human Plasma

Chaube Poonam H., Gandhi Santosh V.*, Deshpande Padmanabh B., Kulkarni Veena G.

Dept. of Pharmaceutical Analysis, A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Pune -411 001, MH, India

*Corresponding Author E-mail: santoshvgandhi@rediffmail.com

Online published on 3 April, 2013.

Abstract

A new simple, accurate, and precise densitometric method for determination of Paracetamol and Etoricoxib in spiked human plasma has been developed and validated. A Simple precipitation method was carried out by using methanol and a known amount of supernatant solution was spotted on precoated silica gel 60 F254 plates using a Camag Linomat V autosampler. The mobile phase selected was Toluene: Dichloromethane: Methanol (6:2.5:1.5 v/v/v) with UV detection at 240 nm. The retention factors for Paracetamol and Etoricoxib were found to be 0.30 ± 0.03 and 0.48 ± 0.05, respectively. The calibration curve was linear in the concentration range 100 to 600 ng per band for both the drugs in human plasma. The limit of quantitation for Paracetamol and Etoricoxib in human plasma was 100 ng for both the drugs and no interference was found from endogenous compounds. The % recovery from human plasma using the precipitation method was found to be 90.46 for Paracetamol and 87.24 for Etoricoxib, respectively. The method provides a direct estimate of the amount of Paracetamol and present in human plasma. The method was validated with respect to linearity, accuracy, precision and stability as per the Guidance for Industry, Bioanlytical Method Validation guidelines.

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Keywords

Paracetamol, Etoricoxib, High Performance Thin Layer chromatography, human plasma.

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