Hepatoprotective Activity of Polyherbal Formulation Containing Some Indigenous Medicinal Plants in Rats
Kandasamy C.S.1,*, E. Mohammed Basil1, P.S. Shimna Thasnim1, Kumar R. Siva1, Gopal V.2, Venkatnarayanan R.1
1Department of Pharmacognosy, R.V.S College of Pharmaceutical Sciences, Sulur, Coimbatore
2Dept. of Pharmacognosy, College of Pharmacy, Mother Theresa Institute of Health Sciences, Pondicherry
*Corresponding Author E-mail: email@example.com
Online published on 3 April, 2013.
Rats and primary cultures of hepatocytes were used as the in vivo and in vitro models to evaluate the hepatoprotective activity of the polyhedral formulation, comprising of Ajowan, Cardamom, Clove, Mace, Nutmeg. Carbon tetrachloride (CCl4) was selected as hepatotoxin. Liv-52 was the reference hepatoprotective agent. In the in vivo study, serum transaminase (SGOT and SGPT), alkaline phosphatase (ALP), total bilirubin, albumin together with total protein and histopathological criteria for the evidences of liver injury. Carbon tetrachloride caused the alterations in all the biochemical parameters and centrilobular necrosis. These biochemical observations were supplemented by histopathological examination of liver sections. Various pathological changes such as architectural intactness, centrilobular observed in rats treated only with carbon tetrachloride, but the groups treated with the carbon tetrachloride and hepatoprotective formulations (Liv-52 and RVSPHF567) were protected to a moderate extent from such pathological changes. It was concluded from the study that the prepared formulation has shown comparable hepatoprotective activity against Carbon tetrachloride induced hepatotoxicity in rats in comparison to Liv-52.
Hepatoprotective activity, Liv-52, Carbon tetrachloride.