Comparative teratogenic and skin whitening effect of herbal extract Glycyrrhiza glabra and Citrus sineus on Zebrafish (Danio rerio) Shaikh Saba1,*, Shaikh Abusufiyan1, Ahmed Ajaz2, Rakhe Nameerah1, Parab Trupti1, Khan Saniya1 1Department of Pharmacology, Anjuman-I-Islam Kalsekar Technical Campus, Navi Mumbai, Maharashtra 2Department of Pharmacognosy, Anjuman-I-Islam Kalsekar Technical Campus, Navi Mumbai, Maharashtra *Corresponding Author E-mail: shaikhsaba66@gmail.com
Online published on 1 June, 2024. Abstract Objective To determine acute toxicity and Synergistic effect of the anti-melanogenic activity of methanolic extract Glycyrrhiza glabra and Citrus sineus on zebrafish. Materials and Method Fish embryo toxicity (FET) was studied as per OECD guidelines 236. The LC50 and maximum non-lethal concentration of both the extracts were calculated. The images of zebrafish larvae exposed to different concentrations of methanolic extracts and their combination were taken by using labomed digital microscope. The skin whitening effect of extract was studied by measuring integrated density of images by using image J software. Result LC50 Value and maximum non-lethal concentration of methanolic extracts of Glycyrrhiza glabra and Citrus sineus were found to be 56.23ppm and 34.7ppm respectively. The result revealed significant reduction in percentage hatchability (p < 0.0014 for glycyrrhiza glabra and p<0.0011 for Citrus sinenus), dose dependant increased in percentage mortality and different developmental morphological abnormalities in zebrafish embryos exposed to the methanolic extracts Glycyrrhiza glabra and Citrus sineus. The synergistic effect of herbal extracts was found to be more effective in reducing the melanin content. Conclusion Very High concentration of Glycyrrhiza glabra and Citrus Sineus showed teratogenic effect in zebrafish embryoes. However, based on OECD aquatic toxicity classification, both the extracts were found to be safe. Our study also revealed synergestic skin whitening effect of both the extracts in zebrafish larvae which might be due to reduction of melatonin content by these extracts. Top Keywords Herbal, Anti-melanogenic, Synergistic, Liquorice, Orange peel powder. Top |