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Research Journal of Pharmacy and Technology
Year : 2022, Volume : 15, Issue : 12
First page : ( 5478) Last page : ( 5482)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.52711/0974-360X.2022.00924

Effect of Carica papaya seed extract on IL -6 and TNF-α in human lung cancer cell lines - An In vitro study

Padmapriya A1, Preetha S1,*, Selvaraj J2, Sridevi G1

1Department of Physiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai.

2Department of Biochemistry, Saveetha Dental college and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai.

*Corresponding Author E-mail: preethas.sdc@saveetha.com

Online Published on 06 March, 2023.

Abstract

Background

Herbal medicine has been used for many years. Medicinal plants are a natural source which has fewer side effects and has an ability to scavenge free radicals. Plants have been used for many centuries to prevent diseases traditionally, because of their pharmacological activities. Carica papaya contains an enzyme called papain which has many pharmaceutical applications and antioxidant activity. Carica papaya contains phytochemicals like flavonoids, alkaloids, sapponents, terpenoids, and amino acids which could be responsible for its medicinal property. The aim of the study is to evaluate the cytotoxic effect of Carica papaya seed and its role on the mRNA expression of IL-6 and TNF-α in human lung cancer cell lines.

Materials and Methods

Cell viability test was done using MTT assay. mRNA expression of IL-6 and TNF-α was done by real-time PCR. The obtained data we analysed statistically by one way analysis of variance and Duncan multiple range tests with graph prism version 5 to analyse the significance. The significance was considered at p<0.05 level in Duncan’s test.

Result

The cells were exposed to different concentrations of Carica papaya (100-500µg/ml) with A549 cells for 48 hours. It showed maximum inhibition of 50% at concentration of 400-500µg/ml. The study showed that Carica papaya seed extract has down-regulated IL-6 and TNF-α mRNA expression at (400-500µg/ml) and it was statistically significant compared to untreated control cells (p< 0.05).

Conclusion

The present study has concluded that cells showed maximum inhibition of 50% at (400µg/ml - 500µg/ml) and thus has anticancer potential by inhibiting mRNA expression of IL-6 and TNF-α in human lung cancer cell lines

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Keywords

Herbal medicine, Carica papaya, Inflammation, Lung cancer cell lines, Innovative technique.

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