The development of the new approaches to the standardization of the Monarda fistulosa L. herbs Kurkin V.A.*, Tsibina A.S. Department of Pharmacognosy, Botany and Phytotherapy, Samara State Medical University, Ulitsa Chapaevskaya 89, Samara, 443099, Russian Federation *Corresponding Author E-mail: Kurkinvladimir@yandex.ru
Online published on 25 August, 2021. Abstract In the present paper are discussed the results of the study on the development of the new approaches to the standardization of the Monarda fistulosa L. herbs. From the herb of the Monarda fistulosa L. there were isolated for the first time isorhoifolin (apigenin-7-O-rutinoside) and linarin (acacetin-7-O-rutinoside) chemical structures of which there were elucidated by means of 1H-NMR-, 1,3C-NMR-, UV-spectroscopy, mass spectrometry and results of chemical transformations. It was determined, that the isolated flavonoids cause the spectral characteristics in the UV spectra of the water-ethanolic extracts from the Monarda fistulosa L. herbs. It was substantiated the expediency of the estimation of the total flavonoids in the herbs of Monarda fistulosa L. calculated on isorhoifolin. As results there was development the method of the quantitative determination of the total flavonoids in the herbs of Monarda fistulosa L. by the using of the differential spectrophotometry at an analytical wavelength of 394 nm. It was determined that the content of the total of flavonoids in the raw materials of the studied plant varies from 4.23±0.08% to 7.68±0.12%. Top Keywords Monarda fistulosa L., Herb, Flavonoids, Isorhoifolin, Linarin, Spectrophotometry, Standardization. Top |