A validated stability indicating UPLC method for simultaneous determination and degradation studies of Sofosbuvir and Velpatasvir in pharmaceutical dosage forms Namratha Sunkara1*, DR. Vijayalakshmi A.2 1Research Scholar, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamil Nadu, India 2School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamil Nadu, India *Corresponding Author E-mail: nimmi.arun58@gmail.com
Online published on 30 April, 2021. Abstract The aim of technique was to develop simple, accurate, rapid, precise and validated Ultra Performance Liquid Chromatographic (UPLC) method for the coinciding analysis of Sofosbuvir and Velpatasvir in pharmaceutical dosage form. The improvement of the strategy was done and also the chromatograms showed smart resolution, retention time, peak response and lowest noise control line ratio by working 50% 0.01N Potassium phosphate monobasic buffer: 50% Acetonitrile at a wavelength of 260nm employing UV detector. The retention time of Sofosbuvir and Velpatasvir was constitute to be 1.188 min and 0.833 min at a flow rate of 0.3ml/min. The present technique was valid for Percent Assay, accuracy, precision, Linearity, LOD and LOQ. The % assay of Sofosbuvir and Velpatasvir was constitute to be 99.66 and 99.76%. The one-dimensionality shown by the medication at a level vary Sofosbuvir (40–240μg/ml) and Velpatasvir (10–60μg/ml) shows regression co-efficient of 0.999 severally. UPLC technique for coinciding estimation of Sofosbuvir and Velpatasvir in Pharmaceutical was developed and valid as per the ICH guidelines. Top Keywords Ultra Performance Liquid Chromatography (UPLC), Acetonitrile, Potassium dihydrogen ortho phosphate, Sofosbuvir and Velpatasvir. Top |