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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2021, Volume : 14, Issue : 3
First page : ( 1542) Last page : ( 1548)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2021.00281.X

Bioactivity of Annona reticulata-An in vitro and in silico approach

Sundar Ranjitha Dhevi V.1, Shankar Saranya1, Ravi Lokesh2, Arunachalam Sathiavelu1*

1School of Bio Sciences and Technology, VIT, Vellore-632014, Tamil Nadu

2Department of Botany, St Joseph's College (Autonomous), Bengaluru, Karnataka, India

*Corresponding Author E-mail: asathiavelu@vit.ac.in

Online published on 30 April, 2021.

Abstract

Aim: The study aimed to analyze the antibacterial, antioxidant and anti-inflammatory potential of two different extracts (Methanol and petroleum ether) of Annona reticulata leaves. Materials and Methods: Dried leaves powder of Annona reticulata was used to study the phytochemical analysis. Methanol and Petroleum ether extracts derived from leaves of A. reticulata were assessed for antioxidant by 2, 2-diphenyl-1-picrylhydrazyl scavenging assay. Agar well diffusion method was used to study the antibacterial activity and in vitro anti-inflammatory activity was evaluated using the albumin denaturation method. And the extracts were subjected to gas chromatography-mass spectrometry (GC-MS) analysis to identify its bioactive components. Results: Qualitative phytochemical screening of leaf powder confirmed the presence of tannins, alkaloids, phenols, flavonoids and saponins. Both the extracts demonstrated significant antibacterial activity against the test organisms, methanol extract showed the highest inhibition zone of about 28mm at 10mg concentration Whereas in petroleum ether extract showed maximum against E.coli with a zone of inhibition of 25mm. Petroleum ether leaf extract demonstrated a maximum anti-inflammatory activity of 83.4% at 150μg/mL concentration and also demonstrated significant antioxidant activity in DPPH assay of 75% at 150μg/mL concentration. In silico docking studies revealed the interaction of ligands from petroleum ether extracts with the seven common antibacterial proteins.

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Keywords

Annona reticulata, Medicinal plant, Antibacterial, Antioxidant, Anti-inflammatory, Auto Dock, Molecular docking.

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