Stability indicating RP-HPLC method for simultaneous estimation of aspirin and prasugrel in combination capsule dosage Devaka Naga Venkata Suresh Kumar1*, Parepalli Yugandhar1, Rao Vallabhaneni Madhusudhan2 1Division of Chemistry, Department of Sciences and Humanities, Vignan's Foundation for Science, Technology and Research University (VFSTRU; Vignan's University), Vadlamudi, Guntur522213, Andhra Pradesh, India. 2School of Chemical Engineering, Vignan's Foundation for Science, Technology and Research University (VFSTRU; Vignan's University), Vadlamudi, Guntur522213, Andhra Pradesh, India. *Corresponding Author E-mail: sureshkumardevaka@gmail.com
Online published on 30 April, 2021. Abstract A rapid, precise, selective and robust stability-indicating RP-HPLC method has been developed for estimation of aspirin (APR) and prasugrel (PSR) in capsule form. The method utilizes waters symmetry C18 (250mm, 4.6mm, 5μm) column, mobile phase consisting of 0.1M of potassium dihydrogen phosphate buffer (pH 3.2) and methanol in the proportion of 65: 35 (v/v) and photodiode array detection at 220nm. The method was linear over a range of 150–450μg/mL (r2-0.999) and 20–60μg/mL (r2-0.999) for APR and PSR, respectively. Relative standard deviation values for APR and PSR during precision studies are smaller than 1.0%. The mean recoveries of APR and PSR were 100.03% and 100.09%, respectively. The forced degradation experiments data confirmed that there was no merging of the peaks of APR and PSR with degradation products. The stability indicating HPLC procedure developedcan be accessibly implemented for regular analysis. Top Keywords RP-HPLC stability indication, Aspirin, Prasugrel, Validation. Top |