Effect of Bryophyllum pinnatum leaves Ethanol extract in TNF-α and TGF-β as candidate therapy of SLE in pristane-induced SLE BALB/c mice model Nurdiana1, Dantara Tri Wahyudi Iman2, Syaban Mokhamad Fahmi Rizki3, Mustafa Siti Asyifa3, Ikhsani Hanifah3, Syafitri Febrinda Esti3, Hapsari Novi Kurnia3, Khoirunnisa Auliya3 1Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia 2Department of Internal Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia 3Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia *Corresponding Author E-mail: farmakodes@gmail.com
Online published on 30 April, 2021. Abstract SLE is an autoimmune disease that has very complex pathogenesis. SLE patients have more sensitive B cells and reduced regulator T cell activity. The increase in activityof B which has play a role in the absorption and presentation of autoantigens to T cells. B cells and T cells will also affect the production of cytokines such as TNF α and TGF-β. We used forty female BALB/c mice, aged 6 until 8 weeks old received a single intraperitoneal injection of 0.5 cc pristane for lupus induction. At 16 weeks after injection, pristane-induced lupus mice were divided into four groups based on the doses received by intragastrically: 10.5mg/kg/day, 21 mg/kg/day, and 42mg/kg/day for 12 weeks. At 38 weeks after injection, all of mice were assessed for inflammation marker. Study results showed that percentages of maturation of B cells, TNF-α, were decreased (p<0.05) and percentages of TGF-β were increased (p>0.05) in Bryophyllum pinnatum leaves ethanol extract-treated mice. Ethanol extract of Bryophyllum pinnatum can inhibit the maturation of B cells so that it can decrease the auto antigen presentation rate in T cells. Bryophyllum pinnatum can also reduce TNF α levels that have been pro-inflammatory agents, whereas Bryophyllum pinnatum can increase TGF-β levels which have the role of anti-inflammatory agent. Top Keywords Systemic Lupus Erythematosus, Bryophyllum pinnatum, Tumor Necrosis Factor α, Tumor Necrosis Factor β. Top |