Topical methotrexate cubosomes in treatment of rheumatoid arthritis: Ex-Vivo and In-Vivo studies Kapoor Karishma2, Pandit Vinay2, Nagaich Upendra1* 1Amity Institute of Pharmacy, Amity University, Sector 125, Noida, UP, 201313. 2Laureate Institute of Pharmacy, Kathog, Kangra, 177101, HP, India. *Corresponding Author E-mail: unagaich@amity.edu
Online published on 30 April, 2021. Abstract Late investigation of complex and shifted parts of rheumatoid joint pain (RA) is prompting the advancement of the more viable focuses for pharmaceutical methodology than it was previously. Current treatment for RA much of the time incorporates the utilization of non-steroidal anti-inflammatory drugs, notwithstanding the extreme antagonistic effects. Local application and consolidation of the medications in cubosomes based formulations may diminish those reactions and improve the efficacy of medications by decreasing the accessibility of them in systemic dissemination and expanding deposition and retention time at the inflammed sites. MTX cubosomes were formulated using Monoolein and Poloxamer-407 and water. The ex vivo skin saturation study was done with the assistance of rat abdominal skin using vertical Franz dispersion cell using PBS and samples were withdrawn and analyzed at defined intervals. In-vivo studies were performed on Female Wistar rats using Bovine type II collagen (CII; Chondrex, Redmond, WA, USA) arrangement in 0.1N acetic acid was emulsified with Freund's adjuvant and the emulsion was imfused subcutaneously at the bottom of the tail. Ex vivo skin permeation using rat abdominal study, primary stage which was quick showed MTX penetration at the first 2h pursued by more slow medication infiltration during the following examined hours. Histopathological studies showed correlated results upon evaluation. Inflammed paw thickness was measured each and there was a considerable decrease in the paw size after the rats were treated with this novel delivery system.: The relative bioavailability of both the formulation was found to be 202.38% and 192.54% respectively, therefore the system was found to be more reliable and non-invasive for topical delivery. Top Keywords Rheumatoid Arthritis, Anti-inflammatory, Methotrexate, Topical, Cubosomes. Top |