Role of medium components for the production of biosurfactant by Achromobacter xylos GSR-21 Reddy Varakala Nikhil12, Reddy Mallu Maheswara12, Siddiqui Nadeem12, Reddy Golamari Siva12*, Sultana Neeha12, Dhakate Divyanshu12, Tripura Ravavarapu Sai12, Reddy N Konda12 1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, India. 2Department of Mathematics, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, India. *Corresponding Author E-mail: gsiva@kluniversity.in
Online published on 30 April, 2021. Abstract Bio surfactants are used to reduce the surface tension between molecules and also plays a major role in microbial enhanced oil recovery and potential uses in territories, which include environmental remediation, agriculture, bio film formation, quorum sensing, textile, prescribed drugs, cosmetics, and the food, oil, and petrochemical industries. In the present paper, optimization of medium components for biosurfactant production by Achromobacter xylos GSR-21 using statistical experimental design was studied. The mineral salt medium requirement for biosurfactant production by Glycolipid Achromobacter xylos was optimized. The important medium components, identified by the initial screening method of Plackett-Burman and response surface quadratic methodology was applied to further optimize bio surfactant production. Regression coefficients were calculated by regression analysis and the model equation was determined. R2 value for biosurfactant (g/L) was calculated as 0.88, and it indicates that the model was well fitted with the experimental results. The important medium components, identified by the initial screening method of Plackett-Burman, were KH2PO4, NH4NO3 and Glycerol (g/l). Response surface quadratic methodology was applied to further optimize biosurfactant production. The maximum biosurfactant production (Achromobacter xylos) (17.9g/L) was predicted at the optimized values of KH2PO4-20 (g/l), glycerol-30 (g/l) and NH4NO3-20(g/l). Surface plots were made, and the obtained mathematical model was verified by performing the experiment with the predicted optimized values, and the yield of bio-surfactant was found to be 9.89g/L. Validation of the predicted model was fitted 98.9% with the experimental results conducted at the optimum conditions. Results of this statistical analysis showed that KH2PO4, glycerol and NH4NO3 had found significant medium components for biosurfactant (Achromobacter xylos) production. Top Keywords Achromobacter xylos GSR-21, PB Design, RSM, MSM Media. Top |