Isolation and characterization of glucanase producing bacteria from forest tree litter
Dwarakanath P.R.1, Rajakumari K.2*, Thiruchelvi R.2
1Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-117, Tamil Nadu, India.
2Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-117, Tamil Nadu, India.
*Corresponding Author E-mail: firstname.lastname@example.org
Online published on 30 April, 2021.
The main objective of the project is to isolate bacteria from Forest soil and characterization of bacteria producing Glucanase. Three different bacteria are isolated from the forest soil. The Bacteria are identified using Biochemical Test. The bacterial strain identified are Bacillus, E. coli and Klebsiella. Two screening for Glucanase producing bacteria are made. The primary screening involves the modified agar diffusion with Congo red assay. The clear white zones are observed in the primary screening. The sample 3 has large white zone when compared to sample 2 and sample 1. The secondary screening involves the Glucanse assay stated by Mendal and Webber. The bacterial sample 3 produced higher concentration of Glucanase than other two bacteria. The sample 3 has produced 0.473mg of Glucose after the conversion of cellulose by the Glucanase enzyme.
Glucanase, Congo red assay, Biochemical test, DNS method by Mandel and Webber.