Phytochemical investigation and In vitro thrombolytic activity of Terminalia pallida brandis leaves
Guguloth Sarvan Kumar12, Malothu Narender1*, Kulandaivelu Umasankar1, Rao Koteswar GSN1, Areti Anka Rao1, Noothi Sadhana3
1KL College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, AP.
2Department of Pharmacology, Vijaya College of Pharmacy, Munaganoor, Hayathnagar, Ranga Reddy-501511.
3B. V. Raju Institute of Technology, Narasapuram, Medak.
*Corresponding Author E-mail: email@example.com
Online published on 30 April, 2021.
In the present work, the preliminary phytochemical study and in vitro thrombolytic activity was conducted for Terminalia pallida Brandis leaves extract. In vitro thrombolytic was evaluated by clot lysis effect of different solvent extracts of Terminalia pallida Brandis leaves. In this study, streptokinase and distilled water were used as positive control and negative control, respectively. In vitro thrombolytic model of methanolic extract showed maximum clot lysis (95.43%±0.697) at test concentration (800μg/ml) in 72 hrs. Hydro alcoholic extract also showed potential clot lysis (90.43%±0.748) at similar test conditions. In addition, other plant extracts such as petroleum ether, ethyl acetate, chloroform extract showed moderate activity at 800 μg/ml concentration i.e., 65.56%±0.984, 70.16%±0.325 and 72.98%±0.985 clot lysis, respectively in 72 hrs. Streptokinase (SK) and water showed clot lysis maximum of 97.26%±0.974 and 41.33%±0.843 respectively. The present study reveals that the methanolic and hydro alcoholic extracts of Terminalia pallida Brandis leaves possesses potential thrombolytic activity.
Terminalia pallida Brandis leaves, Thrombolytic, Clot lysis, Streptokinase.