Inhibitory effect of mosinone-A on immunohistochemical expression of inflammatory and angiogenic markers in 7, 12-dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis
Govindasamy Sugunadevi1*, Kathiresan Suresh2
1Assistant Professor and Head, Department of Biochemistry, Dhanalakshmi Srinivasan College of Arts and Science for Women (Autonomous), Perambalur-621212.
2Associate Professor, Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar.
*Corresponding Author E-mail: email@example.com
Online published on 30 April, 2021.
The present study was investigated to judge the immune histochemical expression of inflammatory and angiogenic markers in DMBA induced hamster buccal pouch carcinogenesis. a complete variety of 24 golden Syrian hamsters were randomized into randomised into four teams of six animals in each. Group I animals were served as untreated control. Groups II and III animals were painted with 0.5% DMBA in liquid paraffin three times per week for 14 weeks on the left buccal pouches. Group III animals were orally administered with Mosinone-A (2mg kg-1 b.wt) starting one week before the exposure to the carcinogen and continued on days alternate to DMBA painting, until the sacrification of the animals. Group IV animals were received Mosinone-A alone throughout the experimental period. Topical application of DMBA for 14 weeks induced buccal pouch carcinomas associated with increased expression of, cytokeratin-8 and iNos where as decreased expression of Cytokeratin. Oral administration of Mosinone-A significantly inhibited the development of HBP carcinomas as revealed by decreased expression of, Cytokeratin-8 and iNos increased expression Cytokeratin. The result of this present study indicates that Mosinone-A will exerts protective effects against DMBA induced buccal pouch carcinogenesis. These findings counsel that Mosinone-A exerts its malignant neoplasm properties by inhibiting cell proliferation and inducement differentiation and caspase mediated cell death.
Oral cancer, Mosinone-A, DMBA iNOS, Cytokeratin, Cytokeratin-8.