Development of UV-Spectrophotometric methods and determination of dissolution profile of Acotiamide Hydrochloride Hydrate
Sahare Kalyani*, Hemke Atul, Dhawale Sandip, Umekar Milind
Department of Pharmaceutical Chemistry, Smt. Kishoritai Bhoyar College of Pharmacy, New Kamptee, Nagpur, Maharashtra-441002, India
*Corresponding Author E-mail: email@example.com
Online published on 30 April, 2021.
The present research work describe development of simple, accurate and reliable UV-Spectrophotometric methods and dissolution test methods for Acotiamide hydrochloride hydratye. The spectrophotometric methods and dissolution test method was performed by subjecting drug and it's formulation to various parameters. The development of UV-Spectrophotometric methods were carried out by using methanol followed by water as a solvent and absorbance measured at selected wavelength 219nm and 286nm for non-zero order And 303.6nm for first order. The correlation coefficient value found to be less than 1 for all developed UV-methods. The proposed method was validated as per ICH guidelines and estimation and recovery in formulation was found to be nearly 100% (% RSD not more than 2%). The dissolution test method was developed on the basis of solubility study of the Acotiamide hydrochloride hydrate different dissolution media of varying pH. The optimized dissolution parameter contains 900ml of Phosphate buffer pH 6.8 as dissolution medium, USP apparatus type-II at 100rpm. The % release obtained around 99.84% in 10 min. Hence the proposed spectrophotometric and dissolution test method can be applied successfully for the quality control of Acotiamide hydrochloride hydrate in marketed tablets.
Acotiamode hydrochloride hydrate (ACT), UV-spectroscopy, Dissolution, Validation.