Phytochemical analysis and investigation of Antimicrobial and Antioxidant potential of the Leaf Extracts of Putranjiva ruxburghi Panda Dibya Sundar1, Padhy Sai Krushna2, Alruwaili Nabil K.1, Gamal Mohammed1, Giri Ranjan Kumar3*, Patro Saroja Kumar3 1College of Pharmacy, Jouf University, Kingdom of Saudi Arabia 2Sidhartha Institute of Pharmacy, Hyderabad, Telangana, India 3Institute of Pharmacy and Technology, Salipur, Cuttack-754202, India *Corresponding Author E-mail: ranjanrifampin@gmail.com
Online Published on 02 March, 2022. Abstract Objectives: Herbs are in use as medicine worldwide from the time immemorial. Many drugs derived from plant, animal or minerals are in use as medicine till date. This is a study on leaf extract of Putranjiva roxburghii Wall. (Euphorbiaceae) involving pharmacognosy, phytochemistry, activity against microbes and oxidation to substantiate its use. The leaf extracts were collected by successive soxhlation using solvents like petroleum ether, chloroform, benzene, acetone & ethanol. Chemicals present in the crude leaf extracts and isolated constituents were analyzed for both quality and quantity followed by TLC, UV-Visible Spectrophotometric analysis, HPTLC, Phytochemical tests and TLC examination confirmed to contain flavonoids in acetone, chloroform, aqueous and ethanol extract. The activity of Petroleum-ether, chloroform, acetone, alcoholic and aqueous extracts against the microbes was assessed by cup plate method. Okada & Okada method was followed to evaluate the DPPH free radical for aging property. The reducing, total antioxidant and peroxide radical for aging action of the extracts were assessed. The study confirmed significant antimicrobial and antioxidant property of the Putranjiva roxburghii Wall leaves may be for the presence of constituents like flavonoids, saponins, Phytosterols, favoring its traditional usage as medicine. Top Keywords Putranjiva roxburghii Wall, Antimicrobial, Antioxidant, Reducing power, Peroxide radical scavenging. Top |