Mesenchymal stem cells prove a significant role in chronic non-healing ulcer progressive healing Athanerey Anjali1, Verma Neha Rani2, Bhargava Piyush3, Patra P K4*, Kumar Awanish1** 1Department of Biotechnology, National Institute of Technology, Raipur, Chhattisgarh. 2Department of Biochemistry, AIIMS, Raipur, Chhattisgarh. 3Department of Biochemistry, Pt JNM Medical College, Raipur, Chhattisgarh. 4Dean, Department of Biochemistry, Pt. J.N.M. Medical College, Raipur, Chhattisgarh. *Corresponding Author E-mail: pkp1964@yahoo.co.in
**drawanishkr@gmail.com, awanik.bt@nitrr.ac.in
Online published on 22 April, 2021. Abstract Severity of non-healing ulcers depends upon both local and systemic factors. Available treatment options like wound dressing, compression therapy, and others have improved clinical outcomes, but chronic wounds continue to exist as the major clinical issues in the patients. As per previous reports, introduction of mesenchymal stem cells has a better treatment option for regenerative therapy. The purpose of this research work was to examine the histopathological and cellular level changes in chronic wound to access whether the application of autologous mesenchymal stem cells improved the wound site environment. Tissue samples from debrided tissue of the chronic wounds were taken before and after the mesenchymal stem cell treatment. Further histopathology and confocal microscopy analysis were also done. Characterization of MSCs was carried out by flow cytometer. We observed that autologous mesenchymal stem cell treatment improved the wound site environment Histopathological and cellular level changes supported the evidence that mesenchymal stem cell treatment reduces the inflammatory damage and facilitating wound area with angiogenesis. Autologous MSCs are positive for CD90, CD105 markers and negative for CD34, CD45 markers. We have found positive results for the treatment of chronic non-healing wounds using mesenchymal stem cells. It is suggested that autologous mesenchymal stem cell treatment has a positive impact on chronic wound healing. Further analysis should be performed to signify some more interactions among cells to facilitate chronic wound healing that would be a silver lining in regenerative medicine. Top Keywords Non-healing ulcer, Chronic, Inflammation, Mesenchymal stem cells, Regenerative therapy. Top |