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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2021, Volume : 14, Issue : 1
First page : ( 344) Last page : ( 350)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2021.00063.9

Exploration of a new Fluoride resistance gene (FRAM gene) in oral Streptococcus mutans MAW

Majeed Atheer Ahmed1*, Atala Mohmmed Lefta1, Mohammed Juma Wsnaa2

1Department of Biology, College of Sciences, University of Baghdad

2Basic Sciences Department, College of Nursing, University of Baghdad

*Corresponding Author E-mail: genomatheer@yahoo.com

Online published on 22 April, 2021.

Abstract

In the present investigations pretended one highly fluoride ion (F) tolerant bacterial strains like Streptococcus mutans MAW was isolated from Iraqi dental patients. The study focuses on a new bacterial fluoride resistance gene that contribute to each of the main virulence defenses to surviving in the oral cavity. The study showed same bacteria was developed fluoride resistance ways using model from hypothetic genes over lapping kind, it confirmed as SNARE-associated domain family genes of cell wall. Material and methods: It was detected by the potential function resistant at 2000 ppm/ml sodium fluoride, which appeared highest level of resistance among four gradient levels of concentrations. The amplification was identified the hypothetic gene of fluoride-resistant using specific primer. Results: The FRAM gene was sequenced with a length 400 bp comparing with NCBI database. The qRT-PCR results had been appeared high expression of FRAM gene in extra level was 5.25-fold compared with control. The sequencing analysis led to detect a new mutant gene for resistance has identity 49% in NCBI database. The result submitted a new Streptococcus mutans MAW (Gen Bank Acc. LC431527) was correlated in genetic distance neighborhood with other fluoride resistance genes of NCBI strains. Conclusion: It was concluded that fluoride exporter confers fluoride resistance to oral S. mutans MAW. The sequencing analysis was able to explain genetic converting in chromosome lead to a fluoride-resistant strain. These explorations can providea new insights into a new mechanism of microbial F+ resistance mutations.

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Keywords

Streptococcus mutans MAW, Fluoride Resistance Genes, SNARE-associated domain family genes, QRT-PCR.

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