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Year : 2021, Volume : 14, Issue : 1
First page : ( 337) Last page : ( 343)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2021.00062.7

Microbiological evaluation of clean rooms in pharmaceutical industries and molecular characterization of microbial isolates

Ramaiyan Gunaseelan1*, Thirumoorthy Viswanathan2

1Research and Development Centre, Bharathiar University, Coimbatore641046, India.

2Department of Microbiology, L.R.G Govt. Arts College for Women, Tirupur-641 604.

*Corresponding Author E-mail: cvramguns@gmail.com

Online published on 22 April, 2021.


Environmental monitoring in pharmaceutical industry was studied by using settle plate, air sampling, surface monitoring and personnel monitoring. These methods are commonly employed to collect the samples in pharmaceutical industry. The collected samples were incubated and predominant bacteria were isolated based on the colony morphology and identified by molecular characterisation. The PCR sequenced bi-directionally for seven isolates i.e, Micrococcus luteus, Bacillus sp, Bacillus Sp. Strain S3Sr84, Kocuria sp. LWYT2000(2016), Staphylococcus sp. Bca53(2016), Brevibacterium sanguinis strain CF52, Staphylococcus hominis strain HB14. Environmental monitoring statistical trend data will provide the process control and environmental isolate must be identified to understand their disinfectant susceptibility.



Environmental monitoring, Molecular Characterization, 16S rDNA, Phylogenetic tree, Disinfectant Validation.


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