Effect of solvent on yield, phytochemicals and in vitro antioxidant potential of Rhododendron arboreum
Sharma Shefali1, Chaudhary Rupali1, Rolta Rajan1, Sharma Nitin2, Sourirajan Anuradha1, Dev Kamal1, Kumar Vikas1*
1Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, PO Sultanpur, Distt. Solan-173229 (HP), India.
2Department of Biotechnology, Chandigarh College of Technology, CGC Landran, 140307, Mohali, Punjab, India.
*Corresponding Author E-mail: firstname.lastname@example.org
Online published on 22 April, 2021.
Rhododendron arboreum (family-Ericaceae) is one among the medicinally and economically important plant species from Himalayan region. Aim of present study was to study the effect of solvent on extraction yield, phytochemicals and antioxidant potential of various parts of R. arboretum. Extract of various solvents (n-hexane, methanol and water) were prepared using cold maceration method. Spectrophotometric method was used to quantify total phenolic and flavonoid content of different extracts. DPPH, FRAP and ABTS method was used to evaluate antioxidant activity of different extracts of various parts of R.arboreum. Among all solvent extract of different part of R.arboretum, highest extraction yield was observed in aqueous extract of leaf (10.78%) followed by methanolic extract of flowers (10.42%). Methanolic extract of flower showed higher amount of TPC (107.46±8.74 mg/g GAE) and TFC (254.26±29.33 mg/g RE). DPPH, FRAP and ABTS assay showed lowest IC50 value in flower extracts (methanolic and water) and hence validating the utilization of flower of this tree as source of natural antioxidants.
Rhododendron arboretum, Cold maceration, Extraction yield, Antioxidant activity.