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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2021, Volume : 14, Issue : 1
First page : ( 153) Last page : ( 161)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2021.00027.5

Enhanced production of an Anti-Cancer pigment from Bacillus endophyticus JUPR15: Single factor system Vs RSM

Venkatachalam Prerana, Nadumane Varalakshmi Kilingar*

Department of Biotechnology, School of Sciences, JAIN (Deemed-to-be University), 18/3, 9th Main, 3rd block, Jayanagar, Bengaluru-560011, India.

*Corresponding Author E-mail: kn.varalakshmi@jainuniversity.ac.in

Online published on 22 April, 2021.

Abstract

As a pink coloured bioactive fraction, B5, produced by B. endophyticus JUPR15 was showing promising anti-cancer properties, response surface methodology (RSM) was employed to enhance its yield. Various carbon, nitrogen, amino acids, vitamins and metal ions along with varying pH, temperature and incubation time were screened independently for enhancing pigment and biomass yield. Central Composite Design (CCD) of RSM was applied to evaluate the optimal conditions for the chosen factors-glycerol, mannitol and malt extract, which were studied at five levels. The R2 value for biomass was 0.9874 and that for pigment yield was 0.9931. The observed responses were in close agreement with the predicted responses. Through CCD, a 14.31-fold (12.02 g/L) increase in biomass and 7.77-fold (202.2 mg/L) increase in pigment yields were achieved as opposed to the 0.84 g/L and 26.03 mg/L of biomass and pigment yields obtained through control conditions. The model was found to be significant (P<0.01) for both the responses. Besides this, the addition of mannitol alone gave 191.36 mg/L of pigment, closer to that obtained via optimized conditions. It can be concluded that both single factor and RSM can be employed to increase the anti-cancer pigment production.

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Keywords

B. endophyticus, Pink pigment, Anti-cancer, Single factor, Central composite design.

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