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Year : 2021, Volume : 14, Issue : 1
First page : ( 143) Last page : ( 145)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2021.00025.1

Simultaneous determination of Trovafloxacin and Marbofloxacin in equine serum and human plasma samples using previously validated HPLC Method

Dr Mahmood Adnan Hussein*

Clinical Pharmacology, PhD The Medical Technical Institute of Baghdad, The Middle Technical University, Iraq

*Corresponding Author E-mail: adnan.mahmood@uqconnect.edu.au, dradnan@imt.edu.iq, dr.adnan73@mtu.edu.iq

Online published on 22 April, 2021.


A previously validated HPLC method was applied for the simultaneous determination of marbofloxacin (MBX) and trovafloxacin (TVX) in equine serum and human plasma. Samples have been precipitated with 20% HClO4 and drugs were separated using a gradient mobile phase system containing of 17.5mM of Monosodium phosphate (NaH2PO4) and 1.5mM aqueous solution of tetrabutylammonium hydroxide (C16H37NO), pH 3 (A) and 50% acetonitrile (C2H3N) and 50% methanol (B). Antibiotics were determined through UV detection at 293 and 270nm. The quantification limits for TVX and MBX were 10ng/ml and 2ng/ml respectively, and the retention times were 6.63 and 4.93 min respectively for both compounds. Over a concentration range of 10–50, 000ng/ml for both antibiotics, the calibration curves were linear. No interacted signals were detected in both horse serum and human plasma. The linearity, precision and accuracy of the assay were estimated from spiked horse serum. The study suggested the use of this method in potential pharmacokinetics studies for selected matrices.



Human plasma, Horse serum, HPLC, Marbofloxacin, Trovafloxacin.


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