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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2021, Volume : 14, Issue : 1
First page : ( 129) Last page : ( 135)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2021.00023.8

Soluble 1: 1 Stoichiometry curcumin binary complex for potential apoptosis in human colorectal adenocarcinoma cells (SW480 and Caco-2 cells)

Mohamed Muthu J1*, Ahmad Fazil2, Kishore Narra1, Al-Subaie Abeer M.3

1Department of Pharmaceutical Technology, BIT Campus, Anna University, Tiruchirappalli620024, Tamil Nadu, India

2Department of Anesthesia Technology, College of Applied Medical Sciences in Jubail, Imam Abdulrahman Bin Faisal University, P.O. Box 4030, Jubail, Saudi Arabia

3Department of Clinical laboratory Sciences, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, Saudi Arabia

*Corresponding Author E-mail: jmuthumohamed@gmail.com

Online published on 22 April, 2021.

Abstract

This study investigates the solid dispersion (SD) of curcumin (CMN) enhance the solubility, which can be employed for the treatment of colorectal cancer (CRC) greater to pure CMN. Solid dispersion (SD) prepared by a hot melt method using CMN with several carriers of poloxamers (P-407 and P-188), gelucire 50/13 (GLR) and mannitol (MNT). Prior, phase solubility studies were performed with drug and carriers. The SD characterized by in vitro drug release and novel dyeing test. Additionally, the cytotoxicity and apoptosis studies resolved to utilize the colorectal adenocarcinoma cell lines. The result showed that CMN-P-407 complex produced significant properties towards solubility (318±14.46 fold) and dissolution (91±0.43% at 30 min). The IC50 value for complex found to be 74 and 52μM/mL, while that for pure CMN ranged from 146 and 116μM/mL on the SW480 and Caco-2 cells respectively. Apoptosis study revealed that the cells are undergoing cell death by apoptosis and the small number of necrosis. The profound efficiency of soluble 1: 1 stoichiometry curcumin binary complex (CMN-P-407 SD) indicated its potential application for CRC treatment by showing a higher capability of inhibiting cell growth compared to that of pure CMN.

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Keywords

Curcumin, Phase solubility studies, Colorectal cancer, MTT assay, Hoechst staining.

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