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Year : 2021, Volume : 14, Issue : 1
First page : ( 67) Last page : ( 74)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2021.00013.5

A Novel method of CFIA/Merging zones technique for assay of Doxycycline in bulk and pharmaceutical preparation depending on Azo dye formation

Tawfeeq Assaf H.1, Qassim Bushra B.2

1Ph D. Student, Department of Chemistry, College of Science, University of Baghdad, Iraq

2 Prof. Analytical Chemistry, Department of Chemistry, College of Science, University of Baghdad, Iraq

*Corresponding Author E-mail: assaf.hameed@gmail.com

Online published on 22 April, 2021.


A simple, automated and sensitive flow-injection analysis/merging zones technique (MZ-FIA) method was developed for the determination of doxycycline hyclate (DOX) in pure and pharmaceutical formulations. This method Involved the reaction of AzoAnthranilic Acid with DOX to produce an orange-yellowish dissolved complex which has maximum absorbance at 468nm. The method was sensitive, precise and accurate were the limit of detection was less than (0.3μg/ml) and the RSD% was less than 1% and Recovery was 98–100%. Various chemical and physical conditions that affected the reaction have been studied. The calibration curve was rectilinear within the concentration range 5–125μg/mL with a sample through put of 80 sample/hour. The proposed procedure was applied successfully for the estimation of DOX and the results obtained were favorably compared with those given by a reference method of British Pharmacopeia, and there was no significant difference between the obtained results, regarding accuracy and precision at the 95% confidence level.



Azo dye, CFIA, Spectrophotometric detection, Doxycycline H2O, Anthranilic Acid.


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