In vitro Cytotoxic studies of Saraca asoca bark extracts on HT-29 cancer cell Line
*Corresponding Author E-mail: firstname.lastname@example.org
Asoka-Saraca asoca (Roxb.) de Wilde is one of the traditional plants with well-known medicinal values prescribed widely to treat haematuria, dyspepsia, fever, tumor, burning sensation, visceromegaly, colic, ulcers, menorrhagia, metropathy leucorrhoea, skin infection along with many other pharmacological activities. As globally, we look towards the use of non-toxic plant based products with conventional medicinal usage and the developing countries depends on herbal medicines for their primary health care needs. And so, the aim of this study is to explore the the in vitro cytotoxicity of the bark extracts of Saraca asoca on HT-29 cancer cell line using MTT and SRB assays. The results show a significant inhibition percentage on HT-29 cancer cell line was observed in both assays. In MTT assay the IC50 values was 174.1 and 163.2μg/ml on alcohol and aqueous bark extracts, respectively. And the SRB assay shows the IC50 values 94.76 and 200.5μg/mL on the crude ethanolic and aqueous bark extracts, respectively. Thus, both MTT and SRB assays can be used for cytotoxic screening of Saraca asoca herb towards the development of modern drug.
MTT assay, SRB assay, HT-29 cancer cell line, IC50 value, Cytotoxic activity.