A bioactive fraction from Turbinaria ornata inducing apoptosis via cell cycle arrest Shabana Parveen, Varalakshmi K Nadumane* Department of Biotechnology, School of Sciences, Jain (Deemed-to-be- University), Bengaluru, India *Corresponding Author E-mail: kn.varalakshmi@jainuniversity.ac.in
Online published on 16 September, 2020. Abstract Cancer is one of the most challenging diseases in both the developed and developing countries and is the second leading cause for the death of people. Finding a therapeutic compound from natural sources to combat cancer is a fascinating area of research for cancer biologists across the globe. Marine macro algae hold promise towards this search as they have an extensive range of bioactivities. They serve as inexhaustible resources of bioactive secondary metabolites with the bioactivities ranging from anti diabetic, anti oxidant, anti microbial, anti inflammatory and anti tumour activity. The present study was aimed at evaluating the anti proliferative activity of the marine alga, Turbinaria ornata (TO), against the cervical cancer (HeLa), breast cancer (MCF-7) and Liver cancer (HepG2) cell lines. The methanol extract of TO inhibited 50% of the cancer cells at 100μg/ml of concentration. The extract was partially purified using different combinations of solvents using thin layer chromatography. The bioactive fraction TF4 induced apoptosis in treated cancer cells which was confirmed through caspase enzyme activity, DNA fragmentation analysis and cell cycle analysis by flow cytometry. The presence of a bioactive compound, 2- phenoxy ethanol was indicated from GC-MS analysis and it can be concluded that the presence 2- phenoxy ethanol could be the reason for the anti cancer activity of T. ornata. Top Keywords Turbinaria ornata, Apoptosis, Caspase enzyme, Cell cycle arrest, DNA fragmentation, LDH. Top |