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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 9
First page : ( 4158) Last page : ( 4164)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00734.9

Methanol and ethyl acetate extracts of red fruit (Pandanus conoideus Lam.) short-red showed high of phenolic and radicals scavenging activities

Maria Matoetina Suprijono1,2,*, Sujuti Hidayat3, Kurnia Dikdik4, Widjanarko Simon Bambang5

1Department of Food Technology, Faculty of Agricultural Technology, Widya Mandala Catholic University Surabaya, Jl. Dinoyo 42–44, Surabaya, 60265, Indonesia

2Postgraduate Program in Agriculture Science, Major in Agricultural Product Technology, Brawijaya University Malang Indonesia, Jl. Veteran, Malang, East Java, Indonesia, 65145

3Faculty of Medicine, Brawijaya University Malang Indonesia, Jl. Veteran, Malang, East Java, Indonesia, 65145

4Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjajaran University Bandung Indonesia, Jl. Raya Bandung Sumedang KM 21 Hegarmanah, Jatinangor, Sumedang, Indonesia, 45363

5Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University Malang Indonesia, Jl. Veteran, Malang, East Java, Indonesia, 65145

*Corresponding Author E-mail: m.matoetina@gmail.com

Online published on 16 September, 2020.

Abstract

Many studies done in Red Fruit/RF as an antioxidant source, but there were some controversies because of the abundant fruit variety and growing place. Most researches focus on Red Fruit oil/RFO. In this work, we analyzed the antioxidant activity of Short-Red RF methanol/ME and ethyl acetic extracts/EE with multiple assays, then evaluated the relationship between phenolic content and antioxidant activity then phenolic effectiveness and antioxidant capacity. Total phenolic content/TPC and antioxidant activities (TAA, DPPH, ABTS, RP) were determined. The flavonoid identification was made using LCMS. RACI and PAC were calculated. EE showed significantly higher TPC (20.11μg GAE/ml extract) and scavenging activity for anion and cation radicals than ME (TPC= 9.88μg GAE/ml extract). The correlation between TAA and TPC (R2= 0.999) or TAA and radicals scavenging activity was significantly strong (R2= 0.997–1.000). EE had high and positive RACI (0.71). ME had higher PAC in TAA (1.82) and ABTS scavenging activity (4.75), whereas EE and ME had the same PAC in DPPH scavenging (2.08). The main phenolic identified in the extracts were taxifolin, quercetin, and quercetin-3-glucoside contributed in the radicals scavenging and also ferric ion reducing activity. Based on this study, Short-Red RF extracts are a potential anion and cation radical scavenger.

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Keywords

Red Fruit Short-Red, Antioxidant activity, Cation Radical, Positive RACI.

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