Determination thymol in thyme extract and its pharmaceutical forms by using gas chromatography method
*Corresponding Author E-mail: firstname.lastname@example.org
The objective of this study was developed and validated of a simple, rapid and accurate GC method for quantitative determination of thymol which considers as a chemical marker in thyme extract and in the final formulation by GC-FID chromatography.
Thymol analysis was done by using menthol as internal standard, chromatographic conditions were TRB-5-625 capillary column and its specification 60m×0.25mm i.d0.25μm film thickness and nitrogen as a carrier gas at a flow rate of 0.6mL/min. The oven temperature was programmed between 185°C and 235°C for 16min. The injector and detector temperatures were maintained 290°C. Detection was done by using flame ionization detector FID.
The developed method was validated according to ICH guidelines. The developed method was given accepted linearity which R>0.99 in the concentration range of 1-40mg/10ml of thymol, accuracy with mean recovery values between 98%-102%, precision with relative standard deviation of the calculated concentration was less than 2%.
The results showed that the developed method was effective in determination of thymol in pharmaceutical forms and routine analysis in quality control laboratories simply.
GC-FID, Thymol, Validation, Thyme extract, Chemical marker.