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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 9
First page : ( 4055) Last page : ( 4060)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00717.9

Determination thymol in thyme extract and its pharmaceutical forms by using gas chromatography method

Helaliy Raghad1, Alrouh Fadi2, Trefi Saleh1, Bitar Yaser1,*

1Department of Pharmaceutical Chemistry and Quality Control-Faculty of Pharmacy, University of Aleppo, Syria

2Department of Organic Chemistry-Faculty of Medicine, University of Al-Hawash, Syria

*Corresponding Author E-mail: dr.ybitar@hotmail.com

Online published on 16 September, 2020.



The objective of this study was developed and validated of a simple, rapid and accurate GC method for quantitative determination of thymol which considers as a chemical marker in thyme extract and in the final formulation by GC-FID chromatography.


Thymol analysis was done by using menthol as internal standard, chromatographic conditions were TRB-5-625 capillary column and its specification 60m×0.25mm i.d0.25μm film thickness and nitrogen as a carrier gas at a flow rate of 0.6mL/min. The oven temperature was programmed between 185°C and 235°C for 16min. The injector and detector temperatures were maintained 290°C. Detection was done by using flame ionization detector FID.


The developed method was validated according to ICH guidelines. The developed method was given accepted linearity which R>0.99 in the concentration range of 1-40mg/10ml of thymol, accuracy with mean recovery values between 98%-102%, precision with relative standard deviation of the calculated concentration was less than 2%.


The results showed that the developed method was effective in determination of thymol in pharmaceutical forms and routine analysis in quality control laboratories simply.



GC-FID, Thymol, Validation, Thyme extract, Chemical marker.


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