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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 8
First page : ( 3807) Last page : ( 3812)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00674.5

Ameliorative effect of papain on behavioral and bio-chemical alterations in in-silico and In-vivo models of neuropathic pain in rats

Pokkula Swapna1, Thaakur Santh Rani2, Krishna Vagolu Siva3, Sriram Dharmarajan3,*

1Department of Pharmacology, Browns College of Pharmacy, Khammam-507305, Telangana, India

2Division of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati-517502, Andhra Pradesh, India

3Department of Pharmacy, Birla Institute of Technology and Science - Pilani, Hyderabad Campus, Jawahar Nagar, Hyderabad-500078, Telangana, India

*Corresponding Author E-mail: drsanthrani@gmail.com

Online published on 16 September, 2020.

Abstract

The present study investigated the ameliorative effect of Papain (PN) on N-methyl D-aspartate (NMDA) receptor down-regulation pathway by using in-silico studies and on oxidative stress in-vivo model of neuropathic pain in rats via partial sciatic nerve ligation (PSNL). In-silico molecular docking study of Papain with NMDA receptors was carried out especially with active pocket of NR2B to know its binding affinity. In in-vivo studies, briefly the rats were randomly divided into sham, vehicle, Pregabalin (standard) and PN treated groups. PSNL was carried out in vehicle and PN treated rats but not in sham and PN (100) treatment control group. PN 50 and 100 mg/kg was administered for a period of 21 days after PSNL. Behavioral alterations were assessed by foot deformity score, cold-allodynia and motor co-ordination tests. On 21st day after behavioral assessment, the rats were sacrificed; sciatic nerve was collected for biochemical estimations of superoxide dismutase (SOD), catalase (CAT), lipid peroxide levels (MDA) and glutathione reductase (GR) levels. In-silico studies revealed that affinity of ligand PN with the NR2B (NMDA) receptor showed best antagonistic effects compared to standard pregabalin. From In-vivo studies, it was observed that PN was significantly (p<0.001) attenuated PSNL induced in behavior and biochemical changes indicating its neuroprotection through antioxidant and antiglutaminergic mechanisms.

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Keywords

Papain, In-silico docking studies, NR2B, In-vivo studies, Neuropathic pain, Oxidative stress.

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