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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 8
First page : ( 3648) Last page : ( 3652)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00645.9

Nephroprotective activity of okra pods extract (Abelmoschus esculentus L.) in sodium nitrite-induced mice

Wahyuningsih Sri Puji Astuti*, Atika Baiq Naili Dewi, Sajidah Elma Sakinatus, Winarni Dwi

Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya, 60115, Indonesia

*Corresponding Author E-mail: sri-p-a-w@fst.unair.ac.id

Online published on 16 September, 2020.


This aim study was conducted to determine the effect of antioxidants from okra pods methanol extract (OPM) in sodium nitrite-induced mice based on total phenolic and flavonoid test, superoxide dismutase (SOD) activity, catalase (CAT) activity, levels of blood urea nitrogen (BUN) and creatinine (Cre), and kidney histology. Okra pods were extracted using methanol solvent. Thirty adult male BALB/c mice (8–10 weeks, ±30g) were divided into six groups: normal control, negative control (sodium nitrite exposure), and treatment group (sodium nitrite exposure and administration of OPM at doses of 50, 100, 200, and 400mg/kg BW). These mice were exposed to sodium nitrite 50mg/kg BW and were administrated with extract OPM for 19 days through gavage. Subsequently, serum was collected to evaluate the activity of SOD and CAT, the levels of BUN and Cre. Kidneys were removed for histopathology studies. The results revealed that sodium nitrite toxicity induced a significant increase in the levels of BUN, Cre, moreover a significant decrease in the activity of SOD and CAT. Sodium nitrite also altered kidney histopathology (necrosis of tubules) compared to the negative control. However, the administration of OPM significantly reduced kidney necrosis, decreased the levels of BUN and Cre, and also significantly increased the activity of SOD and CAT as normal. From the results of this study was concluded that OPM could be exhibiting nephroprotective effect against sodium nitrite induced nephrotoxicity in mice.



Okra pods, Sodium nitrite, Enzyme antioxidant, Enzyme kidney marker, Kidney histology.


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