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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 7
First page : ( 3300) Last page : ( 3304)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00585.5

Molecular Identification of Trichoderma Isolates from Sugarcane Bagasse Based on Internal Transcribed Spacer (ITS) rDNA

Rukmana Siti1, Ansori Arif N. M.1,2, Kusala Muhammad K. J.1, Utami Ulfah3, Wahyudi Didik3, Mandasari Andita A.4,*

1Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia

2Computational Virology and Complexity Science Research Unit, Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, Indonesia

3Faculty of Science and Technology, UIN Maliki Malang, Malang, Indonesia

4Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Indonesia

*Corresponding Author E-mail: andita_ayu_mandasari@dosen.umaha.ac.id

Online published on 10 August, 2020.

Abstract

This study aimed to identify Trichoderma from sugarcane bagasse based on iinternal transcribed spacer (ITS) rDNA. The sample used was pure isolate of Trichoderma from sugarcane bagasse. DNA samples were isolated using the modified CTAB method. DNA was amplified using the primers ITS1 and ITS4. Interestingly, the results showed that Trichoderma genomic DNA has a concentration of 92.56 mg with a purity of 1.91. The amplicon of Trichoderma DNA is about 600 bp, whereas phylogenetic analysis shows that the sample of Trichoderma from sugarcane bagasse is one group with Trichoderma harzianum, Trichoderma piluliferum, Trichoderma sp. SQR339, Hypocrea nigricans, and Trichoderma sp. NFML CH12 BB. 15, Trichoderma aureoviride, Hypocrea lixii, and Trichoderma BAB-4585.

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Keywords

Internal transcribed spacer (ITS), rDNA, sugarcane bagasse, Trichoderma.

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