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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 7
First page : ( 3193) Last page : ( 3198)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00565.X

Detection of Novel apyrimidinic Endonuclease 1 (APE1) in a sample of Iraqi cervical cancer patients using Immunohistochemistry Technique

Al-Zwaini Yasser Kadhim Hashem1,*, Al-Mugdadi Suhad Faisal Hatem2, Abbas Wassan Abdul Kareem2

1Al-Esraa University College, Iraq

2Mustansiriyah University, College of Pharmacy, Department of Clinical Laboratories Sciences, Iraq

*Corresponding Author E-mail: suhadhatim@uomustansiriyah.edu.iq/orcid.org

Online published on 10 August, 2020.

Abstract

Objective

to identify the expression of DNA repair protein apyrimidinic endonuclease 1 (APE1) in samples of Iraqi cervical cancer patients.

Method

expression of APE1 was detected in 55 cervical tissue samples by immunohistochemistry, divided into two groups; 35 cases detected as invasive cervical cancer and 20 cases considered as control. Immunohistochemistry was achieved and the nuclear and cytoplasm-nuclear level of APE1 was counted by taking into account the ratio of positive cells and intensity.

Results

Present data evaluated the prognostic and alterations in APE1 expression levels according to cervical cancer, nuclear stain was seen in the epithelia of 35 cases (100%) of cervical tumor tissues. Only 29 out of 35 cases (82.85%) shown cytoplasmic and nuclear staining with significant difference (P value = 0.00027) between malignant and normal cervical tissue. APE1 high-level expression was (77.1%) and low expression was (22.8%) in cervical cancer tissues. Age, differentiation and histologic type showed highly significant difference (P= 0.0062, 0.0001 and 0.0001 respectively) between the high and low expression of nuclear APEX1 expression. While lymph vascular invasion (positive vs negative group) showed a significant difference (Chi-Square 4.362, P value = 0.0422*) between the high (16/59.2%) and low (4/50%) nuclear expression of APEX1, while lymph node (positive vs negative group) showed no significant association (P-value= 0.1476) between the high and low expression of nuclear APEX1.

Conclusion

The highly positive expression of APE1 results will help Iraqi patients in their early investigation and diagnosis of cervical cancer as good marker for the metastases of their tumor and help researchers to study the lymphangiogensis status of tumor.

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Keywords

APE1 marker, Cervical cancer, IHC, Nuclear expression, Lymphangiogensis.

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